Frontiers in Cell and Developmental Biology (Sep 2020)
miR-146a-5p Plays an Oncogenic Role in NSCLC via Suppression of TRAF6
- Xiangdong Liu,
- Xiangdong Liu,
- Xiangdong Liu,
- Bo Liu,
- Bo Liu,
- Bo Liu,
- Ruihua Li,
- Fei Wang,
- Fei Wang,
- Fei Wang,
- Ning Wang,
- Ning Wang,
- Ning Wang,
- Maihe Zhang,
- Maihe Zhang,
- Maihe Zhang,
- Yang Bai,
- Yang Bai,
- Yang Bai,
- Jin Wu,
- Jin Wu,
- Jin Wu,
- Liping Liu,
- Liping Liu,
- Liping Liu,
- Dongyu Han,
- Dongyu Han,
- Dongyu Han,
- Zhiguang Li,
- Bin Feng,
- Guangbiao Zhou,
- Shujing Wang,
- Shujing Wang,
- Li Zeng,
- Li Zeng,
- Li Zeng,
- Jian Miao,
- Yiqun Yao,
- Bin Liang,
- Lin Huang,
- Lin Huang,
- Qi Wang,
- Yingjie Wu,
- Yingjie Wu,
- Yingjie Wu,
- Yingjie Wu,
- Yingjie Wu
Affiliations
- Xiangdong Liu
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Xiangdong Liu
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Xiangdong Liu
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Bo Liu
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Bo Liu
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Bo Liu
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Ruihua Li
- Department of Clinical Laboratory, Second Affiliated Hospital of Dalian Medical University, Liaoning, China
- Fei Wang
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Fei Wang
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Fei Wang
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Ning Wang
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Ning Wang
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Ning Wang
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Maihe Zhang
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Maihe Zhang
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Maihe Zhang
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Yang Bai
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Yang Bai
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Yang Bai
- Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, Second Affiliated Hospital of Dalian Medical University, Liaoning, China
- Jin Wu
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Jin Wu
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Jin Wu
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Liping Liu
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Liping Liu
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Liping Liu
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Dongyu Han
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Dongyu Han
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Dongyu Han
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Zhiguang Li
- Center of Genome and Personalized Medicine, Institute of Cancer Stem Cell, Cancer Center, Dalian Medical University, Dalian, China
- Bin Feng
- Department of Biotechnology, Dalian Medical University, Dalian, China
- Guangbiao Zhou
- Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Shujing Wang
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Shujing Wang
- Department of Biochemistry and Molecular Biology, Institute of Glycobiology, Dalian Medical University, Dalian, China
- Li Zeng
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Li Zeng
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Li Zeng
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Jian Miao
- Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, Second Affiliated Hospital of Dalian Medical University, Liaoning, China
- Yiqun Yao
- 0Department of Thyroid and Breast Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, China
- Bin Liang
- 1School of Life Sciences, Yunnan University, Kunming, China
- Lin Huang
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Lin Huang
- 2Department of Pathophysiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
- Qi Wang
- 3Department of Respiratory Medicine, Second Affiliated Hospital of Dalian Medical University, Liaoning, China
- Yingjie Wu
- Institute for Genome Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, China
- Yingjie Wu
- National Center of Genetically Engineered Animal Models for International Research, Dalian Medical University, Dalian, China
- Yingjie Wu
- Liaoning Provence Key Lab of Genome Engineered Animal Models, Dalian Medical University, Dalian, China
- Yingjie Wu
- 4Department of Molecular Pathobiology, New York University College of Dentistry, New York, NY, United States
- Yingjie Wu
- 5Division of Endocrinology, Diabetes and Bone Disease, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States
- DOI
- https://doi.org/10.3389/fcell.2020.00847
- Journal volume & issue
-
Vol. 8
Abstract
Non-small cell lung cancer (NSCLC) is the most deadly cancer in the world due to its often delayed diagnosis. Identification of biomarkers with high sensitivity, specificity, and accessibility for early detection, such as circulating microRNAs, is therefore of utmost importance. In the present study, we identified a significantly higher expression of miR-146a-5p in the serum and tissue samples of NSCLC patients than that of the healthy controls. In parallel, miR-146a-5p was also highly expressed in three human NSCLC adenocarcinoma-cell lines (A549, H1299, and H1975) compared to the human bronchial epithelium cell line (HBE). By dual-luciferase reporter assay and manipulation of the expressions of miR-146a-5p and its target gene, tumor necrosis factor receptor-associated factor 6 (TRAF6), we showed that the functional effects of miR-146a-5p on NSCLC cell survival and migration were mediated by direct binding to and suppression of TRAF6. Overexpression of TRAF6 sufficiently reversed miR-146a-5p-induced cancer cell proliferation, migration, and apoptosis resistance. Our data implied that miR-146a-5p/TRAF6/NF-κB-p65 axis could be a promising diagnostic marker and a therapeutic target for NSCLC.
Keywords
