Acta Scientiarum. Health Sciences (May 2017)

<b>Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes

  • Noala Vicensoto Moreira Milhan,
  • Isabel Chaves Silva Carvalho,
  • Renata Falchete do Prado,
  • Eliandra de Sousa Trichês,
  • Carlos Henrique Ribeiro Camargo,
  • Samira Esteves Afonso Camargo

DOI
https://doi.org/10.4025/actascihealthsci.v39i1.29788
Journal volume & issue
Vol. 39, no. 1

Abstract

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The aim of this study was to evaluate the indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental beta tri-calcium phosphate (experimental β-TCP) compared with two other bone substitutes: bovine hydroxyapatite (HA) (Bio-Oss® - Geistlich) and beta tri-calcium phosphate (β-TCP - Bionnovation). The cell viability and genotoxicity were measured by MTT and MNT assay, respectively. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content, and calcium deposition. The MTT and MNT assay showed that none of the tested materials was cytotoxic nor genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with all the materials were able to induce the osteogenesis and this process was influenced by the period of the cell culture in contact with bone substitutes. Based on the results of this study, it was concluded that this experimental β-TCP appears to be a promising material as a bone substitute.

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