Biologics: Targets & Therapy (Jun 2019)
TRIP6 functions as a potential oncogene and facilitated proliferation and metastasis of gastric cancer
Abstract
Lirong Zhu,1* Xiaodong Xu,2* Yijie Tang,3 Xiaochao Zhu41Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, People’s Republic of China; 2Department of General Surgery, Yancheng First People’s Hospital, Yancheng, Jiangsu, People’s Republic of China; 3Department of Clinical Medicine, Medical College of Nantong University, Nantong, Jiangsu, People’s Republic of China; 4Department of General Surgery, Suqian First People’s Hospital, Suqian, Jiangsu, People’s Republic of China*These authors contributed equally to this work Background: Increasing evidence suggests that TRIP6 has been considered to be aberrantly regulated in several malignancies and involved in tumor growth and metastasis. However, the biological role and prognostic significance of TRIP6 in gastric cancer (GC) still remains unclear.Materials and methods: TRIP6 expression was determined in matched GC tissues and adjacent normal tissues by western blot and real-time PCR. Then, immunohistochemistry was used to detect the expression of TRIP6 in GC patients. Statistical analysis was performed to evaluate the correlation between TRIP6 expression and clinicopathological characteristics and prognosis. Moreover, the effects of TRIP6 on GC cell proliferation and migration were also investigated by using MTT, colony formation and transwell assays.Results: We observed that the expression of TRIP6 was significantly up-regulated in GC tissues and cell ines. Our data indicated that high TRIP6 expression exhibited a significant correlation with poor prognosis for GC patients. Multivariate analysis showed that TRIP6 expression was an independent prognostic factor of the overall survival of GC patients. Furthermore, ectopic expression of TRIP6 promotes cell proliferation and migration in BGC823 cells, whereas knockdown of TRIP6 suppresses cell proliferation and migration in MKN45 cells.Conclusion: These findings demonstrate that TRIP6 exerts an important role in cancer development, which represents a potential prognostic indicator in GC.Keywords: gastric cancer, TRIP6, prognosis
