3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration

Hao Huang; Lei Qiang; Minjie Fan; Yihao Liu; Anchun Yang; Dongbiao Chang; Jinsheng Li; Tong Sun; Yiwei Wang; Ruoyi Guo; Hanjie Zhuang; Xiangyu Li; Tailin Guo; Jinwu Wang; Huan Tan; Pengfei Zheng; Jie Weng

Bioactive Materials (Jan 2024)

3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration

Hao Huang,
Lei Qiang,
Minjie Fan,
Yihao Liu,
Anchun Yang,
Dongbiao Chang,
Jinsheng Li,
Tong Sun,
Yiwei Wang,
Ruoyi Guo,
Hanjie Zhuang,
Xiangyu Li,
Tailin Guo,
Jinwu Wang,
Huan Tan,
Pengfei Zheng,
Jie Weng

Affiliations

Hao Huang: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China
Lei Qiang: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China; Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China; Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR China
Minjie Fan: Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China
Yihao Liu: Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China; Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR China
Anchun Yang: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China
Dongbiao Chang: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China
Jinsheng Li: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China
Tong Sun: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China
Yiwei Wang: Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China
Ruoyi Guo: Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China
Hanjie Zhuang: Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China
Xiangyu Li: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR China; School of Mechanical and Electrical Engineering, Henan University of Technology, Zhengzhou 450001, PR China
Tailin Guo: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China
Jinwu Wang: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR China
Huan Tan: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China; Corresponding author.
Pengfei Zheng: Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China; Corresponding author.
Jie Weng: Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China; Corresponding author.

Journal volume & issue: Vol. 31
pp. 18 – 37

Abstract

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The resection of malignant osteosarcoma often results in large segmental bone defects, and the residual cells can facilitate recurrence. Consequently, the treatment of osteosarcoma is a major challenge in clinical practice. The ideal goal of treatment for osteosarcoma is to eliminate it thoroughly, and repair the resultant bone defects as well as avoid bacterial infections. Herein, we fabricated a selenium/strontium/zinc-doped hydroxyapatite (Se/Sr/Zn-HA) powder by hydrothermal method, and then employed it with polycaprolactone (PCL) as ink to construct composite scaffolds through 3D printing, and finally introduced them in bone defect repair induced by malignant osteosarcoma. The resultant composite scaffolds integrated multiple functions involving anti-tumor, osteogenic, and antibacterial potentials, mainly attributed to the anti-tumor effects of SeO32−, osteogenic effects of Sr2+ and Zn2+, and antibacterial effects of SeO32− and Zn2+. In vitro studies confirmed that Se/Sr/Zn-HA leaching solution could induce apoptosis of osteosarcoma cells, differentiation of MSCs, and proliferation of MC3T3-E1 while showing excellent antibacterial properties. In vivo tests demonstrated that Se/Sr/Zn-HA could significantly suppress tumors after 8 days of injection, and the Se/Sr/Zn-HA-PCLs scaffold repaired femoral defects effectively after 3 months of implantation. Summarily, the Se/Sr/Zn-HA-PCLs composite scaffolds developed in this study were effective for tumor treatment, bone defect repair, and post-operative anti-infection, which provided a great potential to be a facile therapeutic material for osteosarcoma resection.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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