Nature Communications (Dec 2016)

Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences

  • Volker Boehm,
  • Jennifer V. Gerbracht,
  • Marie-Charlotte Marx,
  • Niels H. Gehring

DOI
https://doi.org/10.1038/ncomms13691
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 15

Abstract

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Degradation of messenger RNA is a key regulatory step in controlling eukaryotic gene expression. Here the authors present xrFrag, a molecular tool to interrogate the extent and directionality of mRNA turnover by the detection of stabilized decay intermediates produced by several common decay pathways.