Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

Joseph Rosenbluh; Han Xu; William Harrington; Stanley Gill; Xiaoxing Wang; Francisca Vazquez; David E. Root; Aviad Tsherniak; William C. Hahn

doi:10.1038/ncomms15403

Nature Communications (May 2017)

Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

Joseph Rosenbluh,
Han Xu,
William Harrington,
Stanley Gill,
Xiaoxing Wang,
Francisca Vazquez,
David E. Root,
Aviad Tsherniak,
William C. Hahn

Affiliations

Joseph Rosenbluh: Broad Institute of Harvard and MIT
Han Xu: Broad Institute of Harvard and MIT
William Harrington: Broad Institute of Harvard and MIT
Stanley Gill: Broad Institute of Harvard and MIT
Xiaoxing Wang: Broad Institute of Harvard and MIT
Francisca Vazquez: Broad Institute of Harvard and MIT
David E. Root: Broad Institute of Harvard and MIT
Aviad Tsherniak: Broad Institute of Harvard and MIT
William C. Hahn: Broad Institute of Harvard and MIT

DOI: https://doi.org/10.1038/ncomms15403
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 8

Abstract

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CRISPR-Cas9 has been utilized to screen the genome in loss-of-function studies to identify genetic interactions. Here the authors compare catalytically active and dead Cas9 and observe different off-target effects in a screen for essential genes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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