Inventions (Aug 2018)
Gene Delivery System Using Droplet Injector and Temperature-Controlled Planarian Holder
Abstract
A microinjection system for gene delivery to a planarian was presented with materials widely used by manufacturers. The system consists of a nanoliter droplet generator/injector and a planarian holder. Glass capillary needles were used to consistently generate droplets and to inject droplets into a planarian. The holder provides a low-temperature environment that immobilizes the planarian for injection. Our system was tested and showed successful injections of microbeads and droplets with double-stranded RNA into the planarian. The results demonstrated the capability of our system as an alternative for gene delivery for studying gene functions in planarians or other living objects for regenerative medicine studies.
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