Association between rs174537 FADS1 polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity
Chenxuan Wang,
Jessica Murphy,
Kerri Z. Delaney,
Natalie Khor,
José A. Morais,
Michael A. Tsoukas,
Dana E. Lowry,
David M. Mutch,
Sylvia Santosa
Affiliations
Chenxuan Wang
University of Guelph
Jessica Murphy
Kinesiology and Applied Physiology, Concordia University
Kerri Z. Delaney
Kinesiology and Applied Physiology, Concordia University
Natalie Khor
Kinesiology and Applied Physiology, Concordia University
José A. Morais
McGill University Health Centre
Michael A. Tsoukas
McGill University
Dana E. Lowry
University of Guelph
David M. Mutch
University of Guelph
Sylvia Santosa
Kinesiology and Applied Physiology, Concordia University
Fatty acid desaturase 1 (FADS1) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between FADS1 variants and SAT inflammatory status. To advance this area, we conducted an exploratory study to investigate whether the common rs174537 polymorphism in FADS1 was associated with immune cell profiles in abdominal and femoral SAT in individuals with obesity. FADS1 gene expression and immune cell profiles in SAT depots were assessed by qPCR and flow cytometry, respectively. Although FADS1 gene expression was associated with genotype, no associations were observed with immune cell profiles in either depot. Our study provides additional evidence that rs174537 in FADS1 has minimal impact on inflammatory status in obese SAT.
