Effect of L-Glutamic Acid on the Composition and Morphology of Nanostructured Calcium Phosphate as Biomaterial
Fatah Takabait,
Sergio Martínez-Martínez,
Laila Mahtout,
Zahra Graba,
Pedro J. Sánchez-Soto,
Luis Pérez-Villarejo
Affiliations
Fatah Takabait
Laboratoire de Technologie des Matériaux et de Génie des Procédés (LTMGP), Faculté des Sciences Exactes, Université A. Mira-Béjaïa, Terga Ouzemmour, Béjaïa 06000, Algeria
Sergio Martínez-Martínez
Department of Chemical, Environmental and Materials Engineering, Higher Polytechnic School of Linares, University of Jaén, Campus Científico y Tecnológico, Cinturón Sur s/n, 23700 Linares, Spain
Laila Mahtout
Laboratoire de Technologie des Matériaux et de Génie des Procédés (LTMGP), Faculté des Sciences Exactes, Université A. Mira-Béjaïa, Terga Ouzemmour, Béjaïa 06000, Algeria
Zahra Graba
Laboratoire de Technologie des Matériaux et de Génie des Procédés (LTMGP), Faculté des Sciences Exactes, Université A. Mira-Béjaïa, Terga Ouzemmour, Béjaïa 06000, Algeria
Pedro J. Sánchez-Soto
Institute of Materials Science of Sevilla (ICMS), Joint Center of the Spanish National Research Council (CSIC), University of Sevilla, Isla de la Cartuja, 41092 Seville, Spain
Luis Pérez-Villarejo
Department of Chemical, Environmental and Materials Engineering, Higher Polytechnic School of Linares, University of Jaén, Campus Científico y Tecnológico, Cinturón Sur s/n, 23700 Linares, Spain
Calcium phosphate (CaP) with several chemical compositions and morphologies was prepared by precipitation using aqueous solutions of L-Glutamic acid (H2G) and calcium hydroxide, both mixed together with an aqueous solution (0.15 M) of phosphoric acid. Plate-shaped dicalcium phosphate dihydrate (brushite) particles were obtained and identified at a lower concentration of the solution of the reactants. The Ca/P ratio deduced by EDS was ~1, as expected. The nanoscale dimension of carbonate apatite and amorphous calcium phosphate, with variable Ca/P ratios, were revealed by X-ray diffraction (XRD) and scanning electron microscopy and energy dispersive X-ray spectroscopy analysis (SEM-EDS). They were characterized in medium and high concentrations of calcium hydroxide (0.15 M and 0.20 M). The equilibria involved in all the reactions in aqueous solution were determined. The thermodynamic calculations showed a decrease in the amount of chelate complexes with an increase in pH, being the opposite of [CaPO4−] and [CaHG+]. This fluctuation showed an evident influence on the morphology and polymorphism of CaP particles obtained under the present experimental conditions, with potential use as a biomaterial.
