Introgression of Heterotic Genomic Segments from <i>Brassica carinata</i> into <i>Brassica juncea</i> for Enhancing Productivity
Prashant Vasisth,
Naveen Singh,
Omkar Maharudra Limbalkar,
Mohit Sharma,
Gokulan Dhanasekaran,
Mohan Lal Meena,
Priyanka Jain,
Sarika Jaiswal,
Mir Asif Iquebal,
Anshul Watts,
Kiran B. Gaikwad,
Rajendra Singh
Affiliations
Prashant Vasisth
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Naveen Singh
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Omkar Maharudra Limbalkar
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Mohit Sharma
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Gokulan Dhanasekaran
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Mohan Lal Meena
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Priyanka Jain
Division of Agricultural Bioinformatics, Indian Council of Agricultural Research-Indian Agricultural Statistics Research Institute, New Delhi 110012, India
Sarika Jaiswal
Division of Agricultural Bioinformatics, Indian Council of Agricultural Research-Indian Agricultural Statistics Research Institute, New Delhi 110012, India
Mir Asif Iquebal
Division of Agricultural Bioinformatics, Indian Council of Agricultural Research-Indian Agricultural Statistics Research Institute, New Delhi 110012, India
Anshul Watts
Indian Council of Agricultural Research-National Institute of Plant Biotechnology, New Delhi 110012, India
Kiran B. Gaikwad
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Rajendra Singh
Division of Genetics, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India
Interspecific hybridization resulted in the creation of B. juncea introgression lines (ILs) generated from B. carinata with increased productivity and adaptability. Forty ILs were crossed with their respective B. juncea recipient parents to generate introgression line hybrids (ILHs) and the common tester (SEJ 8) was used to generate test hybrids (THs). Mid-parent heterosis in ILHs and standard heterosis in THs were calculated for eight yield and yield-related traits. Heterotic genomic regions were dissected using ten ILs with significant mid-parent heterosis in ILHs and standard heterosis in THs for seed yield. A high level of heterosis for seed yield was contributed by 1000 seed weight (13.48%) in D31_ILHs and by total siliquae/plant (14.01%) and siliqua length (10.56%) in PM30_ILHs. The heterotic ILs of DRMRIJ 31 and Pusa Mustard 30 were examined using polymorphic SNPs between the parents, and a total of 254 and 335 introgressed heterotic segments were identified, respectively. This investigation discovered potential genes, viz., PUB10, glutathione S transferase, TT4, SGT, FLA3, AP2/ERF, SANT4, MYB, and UDP-glucosyl transferase 73B3 that were previously reported to regulate yield-related traits. The heterozygosity of the FLA3 gene significantly improved siliqua length and seeds per siliqua in ILHs of Pusa Mustard 30. This research proved that interspecific hybridization is an effective means of increasing the diversity of cultivated species by introducing new genetic variants and improving the level of heterosis.