Microsatellites for the highly invasive Cameraria ohridella (Lepidoptera: Gracillariidae) and their amplification in related species

Abstract

A set of six polymorphic microsatellite markers was developed for the highly invasive horse chestnut leaf-mining moth, Cameraria ohridella. For 96 positive clones, six polymorphic microsatellites with conserved flanking regions were identified. The average number of alleles per locus was eight (range from three to 12). Two of these loci showed significant heterozygosity deficits due to null alleles. The remaining four loci did not depart from Hardy-Weinberg equilibrium. In addition, all six loci were amplified for 20 other gracillariid species belonging to eight different genera, including another invasive species Phyllonorycter platani. These are the first microsatellite markers described for a species within the lepidopteran family Gracillariidae.

Keywords

• gracillariidae
• aesculus hippocastanum
• cameraria ohridella
• horse chestnut leafminer
• invasion
• microsatellites
• phyllonorycter platani