Journal of Lipid Research (Nov 2007)

Increased concentrations of circulating vitamin E in carriers of the apolipoprotein A5 gene −1131T>C variant and associations with plasma lipids and lipid peroxidation

  • Isabella Sundl,
  • Montse Guardiola,
  • Gholamali Khoschsorur,
  • Rosa Solà,
  • Joan C. Vallvé,
  • Gemma Godàs,
  • Lluís Masana,
  • Michaela Maritschnegg,
  • Andreas Meinitzer,
  • Nicolas Cardinault,
  • Johannes M. Roob,
  • Edmond Rock,
  • Brigitte M. Winklhofer-Roob,
  • Josep Ribalta

Journal volume & issue
Vol. 48, no. 11
pp. 2506 – 2513

Abstract

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The aim of this study was to investigate the effects of the apolipoprotein A5 (APOA5) 1131T>C gene variant on vitamin E status and lipid profile. The gene variant was determined in 297 healthy nonsmoking men aged 20–75 years and recruited in the VITAGE Project. Effects of the genotype on vitamin E in plasma, LDL, and buccal mucosa cells (BMC) as well as on cholesterol and triglyceride (TG) concentrations in plasma and apolipoprotein A-I (apoA-I), apoB, apoE, apoC-III, and plasma fatty acids were determined. Plasma malondialdehyde concentrations as a marker of in vivo lipid peroxidation were determined. C allele carriers showed significantly higher TG, VLDL, and LDL in plasma, higher cholesterol in VLDL and intermediate density lipoprotein, and higher plasma fatty acids. Plasma α-tocopherol (but not γ-tocopherol, LDL α- and γ-tocopherol, or BMC total vitamin E) was increased significantly in C allele carriers compared with homozygote T allele carriers (P = 0.02), but not after adjustment for cholesterol or TG. Plasma malondialdehyde concentrations did not differ between genotypes. In conclusion, higher plasma lipids in the TC+CC genotype are efficiently protected against lipid peroxidation by higher α-tocopherol concentrations. Lipid-standardized vitamin E should be used to reliably assess vitamin E status in genetic association studies.

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