Episomal vector-based generation of human induced pluripotent stem cell line MUSIi020-A from peripheral blood T-cells

Ponthip Pratumkaew; Sudjit Luanpitpong; Phatchanat Klaihmon; Chanchao Lorthongpanich; Chuti Laowtammathron; Sukanya Meesa; Wichuda Suksomboon; Surapol Issaragrisil

Stem Cell Research (Oct 2022)

Episomal vector-based generation of human induced pluripotent stem cell line MUSIi020-A from peripheral blood T-cells

Ponthip Pratumkaew,
Sudjit Luanpitpong,
Phatchanat Klaihmon,
Chanchao Lorthongpanich,
Chuti Laowtammathron,
Sukanya Meesa,
Wichuda Suksomboon,
Surapol Issaragrisil

Affiliations

Ponthip Pratumkaew: Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand
Sudjit Luanpitpong: Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand; Corresponding author.
Phatchanat Klaihmon: Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand
Chanchao Lorthongpanich: Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand
Chuti Laowtammathron: Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand
Sukanya Meesa: Division of Medical Genetics, Department of Obstetrics & Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Wichuda Suksomboon: Division of Medical Genetics, Department of Obstetrics & Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Surapol Issaragrisil: Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

Journal volume & issue: Vol. 64
p. 102929

Abstract

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Human induced pluripotent stem cell (iPSC) line MUSIi020-A was generated from T cells isolated from peripheral blood of a healthy 37-year-old female and reprogrammed using episomal plasmid vectors. The established transgene-free MUSIi020-A, which retained a normal karyotype, displayed pluripotency as characterized by expression of pluripotency markers and by in vitro spontaneous differentiation toward three embryonic germ layers. This cell line may represent a valuable tool for studying T cell development and a potential cell source for cancer immunotherapy.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal