Fabrication of a hydroxyapatite-PDMS microfluidic chip for bone-related cell culture and drug screening

Qiangqiang Tang; Xiaoyu Li; Chen Lai; Lei Li; Hongkai Wu; Yingjun Wang; Xuetao Shi

Bioactive Materials (Jan 2021)

Fabrication of a hydroxyapatite-PDMS microfluidic chip for bone-related cell culture and drug screening

Qiangqiang Tang,
Xiaoyu Li,
Chen Lai,
Lei Li,
Hongkai Wu,
Yingjun Wang,
Xuetao Shi

Affiliations

Qiangqiang Tang: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China
Xiaoyu Li: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China
Chen Lai: Peking University Shenzhen Institute, Peking University, Shenzhen, 518055, PR China
Lei Li: CAS Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China; Corresponding author. CAS Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China.
Hongkai Wu: Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Yingjun Wang: Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China
Xuetao Shi: Peking University Shenzhen Institute, Peking University, Shenzhen, 518055, PR China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005, Guangzhou, PR China; Corresponding author. School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China.

Journal volume & issue: Vol. 6, no. 1
pp. 169 – 178

Abstract

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Bone is an important part of the human body structure and plays a vital role in human health. A microfluidic chip that can simulate the structure and function of bone will provide a platform for bone-related biomedical research. Hydroxyapatite (HA), a bioactive ceramic material, has a similar structure and composition to bone mineralization products. In this study, we used HA as a microfluidic chip component to provide a highly bionic bone environment. HA substrates with different microchannel structures were printed by using ceramic stereolithography (SLA) technology, and the minimum trench width was 50 μm. The HA substrate with microchannels was sealed by a thin polydimethylsiloxane (PDMS) layer to make a HA-PDMS microfluidic chip. Cell culture experiments demonstrated that compared with PDMS, HA was more conducive to the proliferation and osteogenic differentiation of the human foetal osteoblast cell line (hFOB). In addition, the concentration gradient of the model drug doxorubicin hydrochloride (DOX) was successfully generated on a Christmas tree structure HA-PDMS chip, and the half maximal inhibitory concentration (IC50) of DOX was determined. The findings of this study indicate that the HA-PDMS microfluidic chip has great potential in the field of high-throughput bone-related drug screening and bone-related research.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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