فیزیولوژی ورزش و فعالیت بدنی (Apr 2011)
The effect of acute vitamin C supplementation in response to oxidative factors after sub maximal exercise in normobaric hypoxia
Abstract
Background and aim: Maximal exercise in normoxia results in oxidative stress due to an increase in freeradical Production .This increase has negative effect on energy production during exercise and due tofasting fatigue. However, the effect of a single bout of moderate aerobic exercise performed normobarichypoxia on free radical production, lipid per oxidation and total antioxidant capacity (TAC) remainsinconsistent. In this study, we followed the effect of acute vitamin C supplementation in response tooxidative factors after sub maximal exercise in normobaric hypoxia. Material and method: 11activemale) age: 22.18±2.18 yrs, BMI: 21.99±1.24 kg/m2) was required to exercise on a treadmill at 55% ofVO2mox for 35 minutes hypoxic condition [FiO2 (inspired fraction of O2) =0.14(altitude about 3500meter). The subject received orally ascorbic acid (1000 mg/ two hours before exercise session). Bloodsamples were taken before (fasting) and immediately after exercise. Total antioxidant capacity (TAC),lipid per oxidation products malondialdehyde (MDA) and uric acid were measured using HPLC and/orphotometric detection. The results obtained were analyzed by two-way analysis ofvariance (ANOVA)using SPSS statistics software package (version 16) followed by post-hoc Kolmogroph-Smirnov testwhere F value was significant. Level of significance was set at p ≤0.05. Results: The main findings showthat moderate intensity exercise in the hypoxia position, increased significantly plasma-volume-correctedTAC and uric acid concentration (pooled rest compared with exercise P<0.05); However, vitamin Csupplementation did significantly affect MDA concentration (P<0.05). Discussion: These observationswould suggest that single bout of moderate aerobic exercise in normobaric hypoxia increases oxidant andantioxidant markers in oxidative stress and supplementation vitamin C was an effective preventive agent,which further supports the oxidative character (beta oxidation) versus lipid per oxidation induced byhypoxia.
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