Foliar Application of Melatonin Positively Affects the Physio-Biochemical Characteristics of Cotton (<i>Gossypium hirsutum</i> L.) under the Combined Effects of Low Temperature and Salinity Stress

Abstract

Low temperature and soil salinization during cotton sowing and seedling adversely affect cotton productivity. Exogenous melatonin (MT) can alleviate the damage caused to plants under non-biological stress; thus, applying MT is a means to improve the growth condition of crops under stress. However, achieving this goal requires a thorough understanding of the physiological regulatory mechanisms of MT on cotton seedlings under low temperature and salinity stress. This study could bring new knowledge on physio-biochemical mechanisms that improve the tolerance of cotton seedlings to combined effects of low temperature and salt stress using an exogenous foliar application of MT. The phytotron experiment comprised two temperature levels of cold stress and control and five MT treatments of 0, 50, 100, 150, and 200 μM and two salinity levels of 0 and 150 mM NaCl. Compared with the control treatments (non-salinity stress under cold stress and control), the combined stress of salt and low temperature reduced cotton seedlings’ biomass and net photosynthetic rate (Pn), aggravated the membrane damage, reduced the potassium (K+) content, and increased the sodium (Na+) accumulation in the leaves and roots. Under NaCl stress, exogenously sprayed 50–150 μM MT increased the biomass and gas exchange parameters of cotton seedlings under salt and low temperature combined with salt stress, reduced the degree of membrane damage, and regulated the antioxidant enzyme, ion homeostasis, transport, and absorption of cotton seedlings. The pairwise correlation analysis of each parameter using MT shows that the parameters with higher correlation with MT at cold stress are mainly malondialdehyde (MDA), peroxidase (POD), and catalase (CAT). The highest correlation coefficient at 25 °C is observed between the K+ and Na+ content in cotton seedlings. The conclusion indicates that under salt and low-temperature stress conditions, exogenous application of MT primarily regulates the levels of Pn, superoxide dismutase (SOD), andPOD in cotton seedlings, reduces Na+ and MDA content, alleviates damage to cotton seedlings. Moreover, the most significant effect was observed when an exogenous application of 50–150 μM of MT was administered under these conditions. The current study’s findings could serve as a scientific foundation for salinity and low-temperature stress alleviation during the seedling stage of cotton growth.

Keywords

• cotton seedlings
• ion homeostasis
• foliar melatonin
• membrane damage
• low temperature
• salt stress