Porcine sapelovirus 2A protein induces mitochondrial-dependent apoptosis

Chunxiao Mou; Chunxiao Mou; Chunxiao Mou; Yuxi Wang; Shuonan Pan; Kaichuang Shi; Zhenhai Chen; Zhenhai Chen; Zhenhai Chen

doi:10.3389/fimmu.2022.1050354

Frontiers in Immunology (Nov 2022)

Porcine sapelovirus 2A protein induces mitochondrial-dependent apoptosis

Chunxiao Mou,
Chunxiao Mou,
Chunxiao Mou,
Yuxi Wang,
Shuonan Pan,
Kaichuang Shi,
Zhenhai Chen,
Zhenhai Chen,
Zhenhai Chen

Affiliations

Chunxiao Mou: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
Chunxiao Mou: Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
Chunxiao Mou: Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China
Yuxi Wang: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
Shuonan Pan: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
Kaichuang Shi: Guangxi Center for Animal Disease Control and Prevention, Nanning, Guangxi, China
Zhenhai Chen: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China
Zhenhai Chen: Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, China
Zhenhai Chen: Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China

DOI: https://doi.org/10.3389/fimmu.2022.1050354
Journal volume & issue: Vol. 13

Abstract

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Porcine sapelovirus (PSV) is an emerging pathogen associated with symptoms of enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in swine, resulting in significant economic losses. Although PSV is reported to trigger cell apoptosis, its specific molecular mechanism is unclear. In this research, the cell apoptosis induced by PSV infection and its underlying mechanisms were investigated. The morphologic features of apoptosis include nuclear condensation and fragmentation, were observed after PSV infection. The cell apoptosis was confirmed by analyzing the apoptotic rates, caspase activation, and PARP1 cleavage. Caspase inhibitors inhibited the PSV-induced intrinsic apoptosis pathway and reduced viral replication. Among the proteins encoded by PSV, 2A is an important factor in inducing the mitochondrial apoptotic pathway. The conserved residues H48, D91, and C164 related to protease activity in PSV 2A were crucial for 2A-induced apoptosis. In conclusion, our results provide insights into how PSV induces host cell apoptosis.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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