Genetic characterization analysis of hblA and plc genes of foodborne Bacillus cereus in Yueyang City from 2014 to 2019

Abstract

ObjectiveTo provide a genetic basis for scientific prevention and control of food poisoning caused by Bacillus cereus， the pathogenic characteristics of foodborne Bacillus cereus strains isolated in Yueyang City Hu’nan Province from 2014 to 2019 were understood.MethodsThe virulence genes hemolysin A （hblA） and phospholipase C （plc） were sequenced and amplified from 26 Bacillus cereus strains isolated from catering stores in Yueyang City from 2014 to 2019. Seqman and MEGA X software were used to analyze the nucleotide sequences of hblA and plc genes of Bacillus cereus.ResultsThe homology between the coding product of genes hblA and plc of Bacillus cereus isolated from Yueyang City and corresponding Bacillus cereus protein sequences in GenBank were greater than 93.0%.ConclusionThe hblA and plc genes of Bacillus cereus isolated from Yueyang City have high homology with Bacillus cereus gene sequences in GenBank， indicating relatively closer genetic relationship. This study lays the research foundation for further understanding and scientific prevention and control of food poisoning events caused by Bacillus cereus.

Keywords

• bacillus cereus
• hemolysin hbla
• phospholipase c
• genetic characteristics
• foodborne pathogenic bacteria
• hunan province
• yueyang city