Different sterilization methods for overcoming internal bacterial infection in sunflower seeds

Abstract

During culture of protoplasts in agarose droplets, permanent problem was bacterial infection. It was assumed that the seeds are the origin of infection, so different sterilization methods were tested in order to overcome this problem. Germination, infection of seeds and hypocotyls and their growth were examined. Based on these parameters, the best result was obtained with the combined use of 5% commercial bleach and dry heating at 45°C.

Keywords

• bacterial infection
• seeds
• sterilization
• sunflower
• tissue culture