Genomic analysis based on chromosome-level genome assembly reveals Myrtaceae evolution and terpene biosynthesis of rose myrtle
Ling Yang,
Jingjing Jin,
Shanwu Lyu,
Fangqiu Zhang,
Peijian Cao,
Qiaomei Qin,
Guanghui Zhang,
Chen Feng,
Peng Lu,
Huiguang Li,
Shulin Deng
Affiliations
Ling Yang
Key Laboratory of National Forestry and Grassland Administration On Plant Conservation and Utilization in Southern China & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences
Jingjing Jin
National Tobacco Gene Center, Zhengzhou Tobacco Research Institute of CNTC
Shanwu Lyu
Key Laboratory of National Forestry and Grassland Administration On Plant Conservation and Utilization in Southern China & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences
Fangqiu Zhang
Guangdong Eco-Engineering Polytechnic
Peijian Cao
National Tobacco Gene Center, Zhengzhou Tobacco Research Institute of CNTC
Qiaomei Qin
Guangdong Eco-Engineering Polytechnic
Guanghui Zhang
State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan & the Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University
Chen Feng
Jiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences
Peng Lu
National Tobacco Gene Center, Zhengzhou Tobacco Research Institute of CNTC
Huiguang Li
Key Laboratory of National Forestry and Grassland Administration On Plant Conservation and Utilization in Southern China & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences
Shulin Deng
Key Laboratory of National Forestry and Grassland Administration On Plant Conservation and Utilization in Southern China & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences
Abstract Background Rose myrtle (Rhodomyrtus tomentosa (Ait.) Hassk), is an evergreen shrub species belonging to the family Myrtaceae, which is enriched with bioactive volatiles (α-pinene and β-caryophyllene) with medicinal and industrial applications. However, the mechanism underlying the volatile accumulation in the rose myrtle is still unclear. Results Here, we present a chromosome-level genomic assembly of rose myrtle (genome size = 466 Mb, scaffold N50 = 43.7 Mb) with 35,554 protein-coding genes predicted. Through comparative genomic analysis, we found that gene expansion and duplication had a potential contribution to the accumulation of volatile substances. We proposed that the action of positive selection was significantly involved in volatile accumulation. We identified 43 TPS genes in R. tomentosa. Further transcriptomic and TPS gene family analyses demonstrated that the distinct gene subgroups of TPS may contribute greatly to the biosynthesis and accumulation of different volatiles in the Myrtle family of shrubs and trees. The results suggested that the diversity of TPS-a subgroups led to the accumulation of special sesquiterpenes in different plants of the Myrtaceae family. Conclusions The high quality chromosome-level rose myrtle genome and the comparative analysis of TPS gene family open new avenues for obtaining a higher commercial value of essential oils in medical plants.
