Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research

Erica C. Pandolfi; Ernesto J. Rojas; Enrique Sosa; Joanna J. Gell; Timothy J. Hunt; Sierra Goldsmith; Yuting Fan; Sherman J. Silber; Amander T. Clark

Stem Cell Research (Oct 2019)

Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research

Erica C. Pandolfi,
Ernesto J. Rojas,
Enrique Sosa,
Joanna J. Gell,
Timothy J. Hunt,
Sierra Goldsmith,
Yuting Fan,
Sherman J. Silber,
Amander T. Clark

Affiliations

Erica C. Pandolfi: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Ernesto J. Rojas: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Enrique Sosa: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Joanna J. Gell: Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Department of Pediatrics, Division of Hematology-Oncology, David Geffen School of Medicine, Los Angeles, CA, USA
Timothy J. Hunt: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Sierra Goldsmith: Infertility Center of St Louis, St Luke's Hospital, St, Louis, MO, USA
Yuting Fan: Infertility Center of St Louis, St Luke's Hospital, St, Louis, MO, USA
Sherman J. Silber: Infertility Center of St Louis, St Luke's Hospital, St, Louis, MO, USA
Amander T. Clark: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; Corresponding author at: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA.

Journal volume & issue: Vol. 40

Abstract

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We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDFs) (MZT04) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cells. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using teratomas and PluriTest. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility and infertility.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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