Frontiers in Microbiology (Jul 2021)
Microbiome in Blood Samples From the General Population Recruited in the MARK-AGE Project: A Pilot Study
- Patrizia D’Aquila,
- Robertina Giacconi,
- Marco Malavolta,
- Francesco Piacenza,
- Alexander Bürkle,
- María Moreno Villanueva,
- María Moreno Villanueva,
- Martijn E. T. Dollé,
- Eugène Jansen,
- Tilman Grune,
- Tilman Grune,
- Efstathios S. Gonos,
- Claudio Franceschi,
- Claudio Franceschi,
- Miriam Capri,
- Miriam Capri,
- Beatrix Grubeck-Loebenstein,
- Ewa Sikora,
- Olivier Toussaint,
- Florence Debacq-Chainiaux,
- Antti Hervonen,
- Mikko Hurme,
- P. Eline Slagboom,
- Christiane Schön,
- Jürgen Bernhardt,
- Nicolle Breusing,
- Giuseppe Passarino,
- Mauro Provinciali,
- Dina Bellizzi
Affiliations
- Patrizia D’Aquila
- Department of Biology, Ecology and Earth Sciences (DIBEST), University of Calabria, Rende, Italy
- Robertina Giacconi
- Advanced Technology Center for Aging Research, IRCCS (Scientific Institute for Research, Hospitalization and Healthcare) INRCA National Institute on Health and Science on Ageing, Ancona, Italy
- Marco Malavolta
- Advanced Technology Center for Aging Research, IRCCS (Scientific Institute for Research, Hospitalization and Healthcare) INRCA National Institute on Health and Science on Ageing, Ancona, Italy
- Francesco Piacenza
- Advanced Technology Center for Aging Research, IRCCS (Scientific Institute for Research, Hospitalization and Healthcare) INRCA National Institute on Health and Science on Ageing, Ancona, Italy
- Alexander Bürkle
- Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
- María Moreno Villanueva
- Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
- María Moreno Villanueva
- Department of Sport Science, Human Performance Research Centre, University of Konstanz, Konstanz, Germany
- Martijn E. T. Dollé
- Centre for Health Protection, National Institute for Public Health and the Environment, Bilthoven, Netherlands
- Eugène Jansen
- Centre for Health Protection, National Institute for Public Health and the Environment, Bilthoven, Netherlands
- Tilman Grune
- Department of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), Nuthetal, Germany
- Tilman Grune
- NutriAct-Competence Cluster Nutrition Research Berlin-Potsdam, Nuthetal, Germany
- Efstathios S. Gonos
- National Hellenic Research Foundation, Institute of Biology, Medicinal Chemistry and Biotechnology, Athens, Greece
- Claudio Franceschi
- Department of Experimental, Diagnostic and Specialty Medicine, Alma Mater Studiorum, University of Bologna, Bologna, Italy
- Claudio Franceschi
- 0Institute of Information Technologies, Mathematics and Mechanics, Lobachevsky University, Nizhny Novgorod, Russia
- Miriam Capri
- Department of Experimental, Diagnostic and Specialty Medicine, Alma Mater Studiorum, University of Bologna, Bologna, Italy
- Miriam Capri
- 1Interdepartmental Center, Alma Mater Research Institute on Global Challenges and Climate Change, University of Bologna, Bologna, Italy
- Beatrix Grubeck-Loebenstein
- 2Research Institute for Biomedical Aging Research, University of Innsbruck, Innsbruck, Austria
- Ewa Sikora
- 3Laboratory of the Molecular Bases of Ageing, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
- Olivier Toussaint
- 4Research Unit of Cellular Biology (URBC) Namur Research Institute for Life Sciences (Narilis), University of Namur, Namur, Belgium
- Florence Debacq-Chainiaux
- 4Research Unit of Cellular Biology (URBC) Namur Research Institute for Life Sciences (Narilis), University of Namur, Namur, Belgium
- Antti Hervonen
- 5Medical School, University of Tampere, Tampere, Finland
- Mikko Hurme
- 5Medical School, University of Tampere, Tampere, Finland
- P. Eline Slagboom
- 6Department of Molecular Epidemiology, Leiden University Medical Centre, Leiden, Netherlands
- Christiane Schön
- 7BioTeSys GmbH, Schelztorstr, Esslingen, Germany
- Jürgen Bernhardt
- 7BioTeSys GmbH, Schelztorstr, Esslingen, Germany
- Nicolle Breusing
- 8Department of Applied Nutritional Science/Dietetics, Institute of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany
- Giuseppe Passarino
- Department of Biology, Ecology and Earth Sciences (DIBEST), University of Calabria, Rende, Italy
- Mauro Provinciali
- Advanced Technology Center for Aging Research, IRCCS (Scientific Institute for Research, Hospitalization and Healthcare) INRCA National Institute on Health and Science on Ageing, Ancona, Italy
- Dina Bellizzi
- Department of Biology, Ecology and Earth Sciences (DIBEST), University of Calabria, Rende, Italy
- DOI
- https://doi.org/10.3389/fmicb.2021.707515
- Journal volume & issue
-
Vol. 12
Abstract
The presence of circulating microbiome in blood has been reported in both physiological and pathological conditions, although its origins, identities and function remain to be elucidated. This study aimed to investigate the presence of blood microbiome by quantitative real-time PCRs targeting the 16S rRNA gene. To our knowledge, this is the first study in which the circulating microbiome has been analyzed in such a large sample of individuals since the study was carried out on 1285 Randomly recruited Age-Stratified Individuals from the General population (RASIG). The samples came from several different European countries recruited within the EU Project MARK-AGE in which a series of clinical biochemical parameters were determined. The results obtained reveal an association between microbial DNA copy number and geographic origin. By contrast, no gender and age-related difference emerged, thus demonstrating the role of the environment in influencing the above levels independent of age and gender at least until the age of 75. In addition, a significant positive association was found with Free Fatty Acids (FFA) levels, leukocyte count, insulin, and glucose levels. Since these factors play an essential role in both health and disease conditions, their association with the extent of the blood microbiome leads us to consider the blood microbiome as a potential biomarker of human health.
Keywords
