Spectroscopic Characterization of Bovine, Avian and Johnin Purified Protein Derivative (PPD) with High-Throughput Fourier Transform InfraRed-Based Method
Sara Corneli,
Laura Corte,
Luca Roscini,
Antonella Di Paolo,
Claudia Colabella,
Linda Petrucci,
Giulio Severi,
Monica Cagiola,
Piera Mazzone
Affiliations
Sara Corneli
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via Gaetano Salvemini 1, 06126 Perugia, Italy
Laura Corte
Department of Pharmaceutical Sciences–Microbiology section, University of Perugia, Borgo 20 Giugno 74, 06121 Perugia, Italy
Luca Roscini
Department of Pharmaceutical Sciences–Microbiology section, University of Perugia, Borgo 20 Giugno 74, 06121 Perugia, Italy
Antonella Di Paolo
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via Gaetano Salvemini 1, 06126 Perugia, Italy
Claudia Colabella
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via Gaetano Salvemini 1, 06126 Perugia, Italy
Linda Petrucci
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via Gaetano Salvemini 1, 06126 Perugia, Italy
Giulio Severi
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via Gaetano Salvemini 1, 06126 Perugia, Italy
Monica Cagiola
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via Gaetano Salvemini 1, 06126 Perugia, Italy
Piera Mazzone
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via Gaetano Salvemini 1, 06126 Perugia, Italy
Tuberculins purified protein derivatives (PPDs) are obtained by precipitation from heat treated mycobacteria. PPDs are used in diagnosis of mycobacterial infections in humans and animals. Bovine PPD (PPDB) is obtained from Mycobacterium bovis (Mycobacterium tuberculosis complex), while Avian PPD (PPDA) and Johnin PPD (PPDJ) are extracted, respectively, from Mycobacterium avium and M. avium subsp. paratuberculosis (M. avium complex). PPDB and PPDA are used for bovine tuberculosis diagnosis, while PPDJ is experimentally used in the immunodiagnosis of paratuberculosis. Although PPDs date back to the 19th Century, limited knowledge about their composition is currently available. The goal of our study was to evaluate Fourier Transform InfraRed (FTIR) spectroscopy as a tool to differentiate PPDB, PPDA, and three PPDJs. The results highlighted that the three PPDs have specific profiles, correlated with phylogenetic characteristics of mycobacteria used for their production. This analysis is eligible as a specific tool for different PPDs batches characterization and for the assessment of their composition. The entire PPD production may be efficiently controlled, since the N content of each preparation is related to IR spectra, with a reference spectrum for each PPD and a standardized analysis protocol.
