Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System

Shengde Liu; Dejin Zheng; Jiaosheng Li; Jindong Tian; Liyun Zhong; Xiaoxu Lu

doi:10.1109/JPHOT.2019.2955751

IEEE Photonics Journal (Jan 2020)

Quantitative Phase Imaging During Cell Division Based on Dual-Channel Microscopic Interferometry System

Shengde Liu,
Dejin Zheng,
Jiaosheng Li,
Jindong Tian,
Liyun Zhong,
Xiaoxu Lu

Affiliations

Shengde Liu: ORCiD; Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, China
Dejin Zheng: Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, China
Jiaosheng Li: ORCiD; Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, China
Jindong Tian: ORCiD; Shenzhen Key Laboratory of Micro-Nano Measuring and Imaging in Biomedical Optics, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
Liyun Zhong: Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, China
Xiaoxu Lu: ORCiD; Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, South China Normal University, Guangzhou, China

DOI: https://doi.org/10.1109/JPHOT.2019.2955751
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

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Quantitative phase imaging (QPI) during cell division is implemented by ourhomemade dual-channel microscopic interferometry (DCMI) system.A pair of interferograms with a fixed phase shift of π/2 is simultaneously captured by the DCMI system, and two-step phase demodulation algorithm is employed for phase retrieval. By capturing a sequence of paired-interferograms with the DCMI system, we achieve the dynamic QPIduring cell division, and then the cellular surface area, volume and the ratio of surface area to volume (RSV) and their variations. Both the reliability and stability of the DCMI system are verified. In addition to maintaining the advantages of optical interferometry, this DCMI system is very suitable for dynamic QPI due to its rapid speed of phase retrieval. Importantly, thisDCMI based QPImethod will supply a powerful tool for studying the precise mechanism during cell division, differentiation, apoptosis and other dynamic processes.

Published in IEEE Photonics Journal

ISSN: 1943-0655 (Online)
Publisher: IEEE
Country of publisher: United States
LCC subjects: Technology: Engineering (General). Civil engineering (General): Applied optics. Photonics; Science: Physics: Optics. Light
Website: http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=4563994

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