THE EFFECT OF CRUDE Nigella sativa OIL AGAINST THE ACUTE TOXICITY OF DICLOFENAC SODIUM AND IBUPROFEN ON THE LIVERS OF ALBINO MICE

Abstract

The present research work is an investigation of the effect of Nigella sativa oil against the acute toxicity induced by the drugs diclofenac and ibuprofen on the liver enzymes, body weight and Hepatosomatic index of Swiss albino mice. Thirty-six healthy, adult Swiss albino mice were used to assess diclofenac- and ibuprofen-induced hepatotoxicity and the hepatoprotective effect of N. sativa oil. The animals were divided into the control group and five experimental groups. The animals in the control group were given saline (0.9 percent of NaCl) only, whereas the animals in experimental groups were given single sub-lethal doses of diclofenac, ibuprofen and N. sativa oil alone and together. A significant (p<0.05) reduction in the body weight of the diclofenac- and ibuprofen-treated groups was recorded. The hepatosomatic index showed significant (p<0.05) changes in the combined treated groups. Hepatotoxicity can be confirmed by comparing the significant (p<0.05) and highly significant (p<0.01) increase in liver enzymes in all the treated animals. The hepatoprotective effect of N. sativa oil was confirmed. Key words: diclofenac; ibuprofen; hepatotoxicity; hepatoprotective; N. sativa oil VPLIV OLJA ČRNE KUMINE (Nigella sativa) NA AKUTNO ZASTRUPITEV JETER, POVZROČENO Z NATRIJEVIM DIKLOFENAKOM IN IBUPROFENOM, PRI ALBINO MIŠIH Predstavljena raziskava opisuje učinke olja črne kumine (Nigella sativa) na aktivnost jetrnih encimov, telesno težo in hepatosomatski indeks pri švicarskih albino miših pri akutni zastrupitvi, povzročeni z natrijevim diklofenakom in ibuprofenom. Za oceno škodljivega učinka diklofenaka in ibuprofena na jetra ter zaščitnega učinka olja N. sativa na jetra je bilo testiranih šestintrideset zdravih odraslih miši. Živali so bile razdeljene v kontrolno skupino in v pet poskusnih skupin. Živali v kontrolni skupini so prejele fiziološko raztopino (0,9 % NaCl), medtem ko so dobile živali v poskusnih skupinah različne subletalne odmerke diklofenaka in ibuprofena samostojno ali pa v kombinaciji z oljem črne kumine. Rezultati so pokazali statistično značilno (p < 0,05) znižanje telesne mase pri skupinah mišk, ki so dobivale diklofenak in ibuprofen. Pri vseh skupinah, tretiranih z diklofenakom in iboprufenom, so se pokazale statistično značilne razlike med kontrolno in tretiranimi skupinami, kar potrjuje hepatotoksični vpliv tretiranja. Hepatosomatski indeks je pokazal statistično značilne (p < 0,05) razlike med skupinami, ki so bile tretirane s kombinacijo učinkovin, in skupinami, ki so prejemale tudi olje črne kumine, kar kaže na hepatoprotektivni učinek olja črne kumine. Ključne besede: diklofenak; ibuprofen; hepatotoksičnost; hepatoprotektivnost; olje N. sativa