Increase in the Synthesis of Polyfructan in the Cultures of Chicory “Hairy Roots” with Plant Natural Growth Regulators

Abstract

Experiments were conducted to study the benefit of using the new plant growth regulators (PGRs) Ivin, Emistim, Biolan and Charkor in nutrient medium ½ MS for intensification of biomass growth and the increased synthesis of polyfructan (PF) in the cultures of chicory “hairy roots” (Cichorium intybus L.), obtained by Аgrobacterium rhizogenes-mediated transformation,. The best indexes of increased specific quantities of PF are observed after using Biolan at a concentration of 5.0 μL/L (up to 130 mg/g dry mass of roots) and Emistim at a concentration 2.5 µL/L (up to 220 mg/g dry mass of roots). The greatest stimulation of root growth activity was expressed on using the growth regulators Emistim, Ivin and Charkor, in concentrations between 2.5 and 10.0 µL/L, considerably raising the total quantity of PF: compared with the control the use of regulator Emistim showed a rise of up to 35 times, regulator Ivin showed an increase of up to 28 times and regulator Charkor showed an increase up to 7.0-7.5 times. The results thus obtained definitely prove the benefit of applying these regulators to increase the biomass growth and PF synthesis in the culture of chicory «hairy roots».

Keywords

• plant growth regulators
• chicory "hairy roots"
• polyfructan.