The pH stability of foot-and-mouth disease virus

Hong Yuan; Pinghua Li; Xueqing Ma; Zengjun Lu; Pu Sun; Xingwen Bai; Jing Zhang; Huifang Bao; Yimei Cao; Dong Li; Yuanfang Fu; Yingli Chen; Qifeng Bai; Jie Zhang; Zaixin Liu

doi:10.1186/s12985-017-0897-z

Virology Journal (Nov 2017)

The pH stability of foot-and-mouth disease virus

Hong Yuan,
Pinghua Li,
Xueqing Ma,
Zengjun Lu,
Pu Sun,
Xingwen Bai,
Jing Zhang,
Huifang Bao,
Yimei Cao,
Dong Li,
Yuanfang Fu,
Yingli Chen,
Qifeng Bai,
Jie Zhang,
Zaixin Liu

Affiliations

Hong Yuan: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Pinghua Li: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Xueqing Ma: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Zengjun Lu: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Pu Sun: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Xingwen Bai: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Jing Zhang: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Huifang Bao: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Yimei Cao: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Dong Li: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Yuanfang Fu: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Yingli Chen: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Qifeng Bai: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University
Jie Zhang: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Zaixin Liu: State Key Laboratory of Veterinary Etiological Biology, OIE/China Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences

DOI: https://doi.org/10.1186/s12985-017-0897-z
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 10

Abstract

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Abstract ᅟ This review summarized the molecular determinants of the acid stability of FMDV in order to explore the uncoating mechanism of FMDV and improve the acid stability of vaccines. Background The foot-and-mouth disease virus (FMDV) capsid is highly acid labile and tends to dissociate into pentameric subunits at acidic condition to release viral RNA for initiating virus replication. However, the acid stability of virus capsid is greatly required for the maintenance of intact virion during the process of virus culture and vaccine production. The conflict between the acid lability in vivo and acid stability in vitro of FMDV capsid promotes the selection of a series of amino acid substitutions which can confer resistance to acid-induced FMDV inactivation. In order to explore the uncoating activity of FMDV and enhance the acid stability of vaccines, we summarized the available works about the pH stability of FMDV. Main body of the abstract In this review, we analyzed the intrinsic reasons for the acid instability of FMDV from the structural and functional aspects. We also listed all substitutions obtained by different research methods and showed them in the partial capsid of FMDV. We found that a quadrangle region in the viral capsid was the place where a great many pH-sensitive residues were distributed. As the uncoating event of FMDV is dependent on the pH-sensitive amino acid residues in the capsid, this most pH-sensitive position indicates a potential candidate location for RNA delivery triggered by the acid-induced coat disassociation. Short conclusion This review provided an overview of the pH stability of FMDV. The study of pH stability of FMDV not only contributes to the exploration of molecule and mechanism information for FMDV uncoating, but also enlightens the development of FMDV vaccines, including the traditionally inactivated vaccines and the new VLP (virus-like particle) vaccines.

Published in Virology Journal

ISSN: 1743-422X (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: http://virologyj.biomedcentral.com

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