Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles

Christopher A. Gaulke; Emily R. Schmeltzer; Mark Dasenko; Brett M. Tyler; Rebecca Vega Thurber; Thomas J. Sharpton

doi:10.1128/mSystems.00440-21

mSystems (Oct 2021)

Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles

Christopher A. Gaulke,
Emily R. Schmeltzer,
Mark Dasenko,
Brett M. Tyler,
Rebecca Vega Thurber,
Thomas J. Sharpton

Affiliations

Christopher A. Gaulke: ORCiD; Department of Microbiology, Oregon State University, Corvallis, Oregon, USA
Emily R. Schmeltzer: ORCiD; Department of Microbiology, Oregon State University, Corvallis, Oregon, USA
Mark Dasenko: Center for Quantitative Life Sciences, Oregon State University, Corvallis, Oregon, USA
Brett M. Tyler: Center for Quantitative Life Sciences, Oregon State University, Corvallis, Oregon, USA
Rebecca Vega Thurber: Department of Microbiology, Oregon State University, Corvallis, Oregon, USA
Thomas J. Sharpton: Department of Microbiology, Oregon State University, Corvallis, Oregon, USA

DOI: https://doi.org/10.1128/mSystems.00440-21
Journal volume & issue: Vol. 6, no. 5

Abstract

Read online

Metagenomic library preparation methods and sequencing technologies continue to advance rapidly, allowing researchers to characterize microbial communities in previously underexplored environmental samples and systems. However, widely accepted standardized library preparation methods can be cost-prohibitive.

Published in mSystems

ISSN: 2379-5077 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msystems

About the journal