Supporting data of spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo
Gustavo A. Higuera,
Hugo Fernandes,
Tim W.G.M. Spitters,
Jeroen van de Peppel,
Nils Aufferman,
Roman Truckenmueller,
Maryana Escalante,
Reinout Stoop,
Johannes P van Leeuwen,
Jan de Boer,
Vinod Subramaniam,
Marcel Karperien,
Clemens van Blitterswijk,
Anton van Boxtel,
Lorenzo Moroni
Affiliations
Gustavo A. Higuera
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Hugo Fernandes
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Tim W.G.M. Spitters
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Jeroen van de Peppel
Erasmus Medical Center, Internal Medicine, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands
Nils Aufferman
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Roman Truckenmueller
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Maryana Escalante
Biophysical Engineering Group, Mesa+ Institute for Nanotechnology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands
Reinout Stoop
TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, The Netherlands
Johannes P van Leeuwen
Erasmus Medical Center, Internal Medicine, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands
Jan de Boer
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Vinod Subramaniam
Biophysical Engineering Group, Mesa+ Institute for Nanotechnology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands
Marcel Karperien
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Clemens van Blitterswijk
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
Anton van Boxtel
Systems and Control Group, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands
Lorenzo Moroni
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands
This data article contains seven figures and two tables supporting the research article entitled: spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo [1]. The data explain the culture of stromal cells in vitro in three culture systems: discs, scaffolds and scaffolds in a perfusion bioreactor system. Also, quantification of extracellular matrix components (ECM) in vitro and staining of ECM components in vivo can be found here. Finally the quantification of blood vessels dimensions from CD31 signals and representative histograms of stanniocalcin-1 fluorescent signals in negative controls and experimental conditions in vivo are presented.
