Generation of induced pluripotent stem cells from ADHD patients who do or do not respond to Methylphenidate treatment

Cristine Marie Yde Ohki; Natalie Monet Walter; Michelle Rickli; Cedric Iseli; Anna Maria Werling; Christian Döring; Belén Rubio; Per Hoffmann; Stefan Herms; Susanne Walitza; Edna Grünblatt

Stem Cell Research (Dec 2024)

Generation of induced pluripotent stem cells from ADHD patients who do or do not respond to Methylphenidate treatment

Cristine Marie Yde Ohki,
Natalie Monet Walter,
Michelle Rickli,
Cedric Iseli,
Anna Maria Werling,
Christian Döring,
Belén Rubio,
Per Hoffmann,
Stefan Herms,
Susanne Walitza,
Edna Grünblatt

Affiliations

Cristine Marie Yde Ohki: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Natalie Monet Walter: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Michelle Rickli: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Cedric Iseli: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Anna Maria Werling: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Christian Döring: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Belén Rubio: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Per Hoffmann: Human Genomics Research Group, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland; Institute of Human Genetics, University of Bonn, School of Medicine & University Hospital Bonn, 53105 Bonn, Germany
Stefan Herms: Human Genomics Research Group, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland; Institute of Human Genetics, University of Bonn, School of Medicine & University Hospital Bonn, 53105 Bonn, Germany
Susanne Walitza: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland
Edna Grünblatt: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland; Corresponding author at: Department of Child and Adolescent Psychiatry & Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Wagistrasse 12, 8952 Schlieren, Switzerland.

Journal volume & issue: Vol. 81
p. 103546

Abstract

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As a neurodevelopmental multifactorial disorder whose prevalence has been increasing worldwide, attention-deficit hyperactivity disorder (ADHD) is considered a public health concern. Methylphenidate (MPH) is the drug of choice for ADHD; however, not all patients respond fully to this treatment. Therefore, exploring the underlying molecular mechanisms involved in ADHD and potential novel therapeutic targets is crucial. Here, we generated induced pluripotent stem cells (iPSCs) from Peripheral Blood Mononuclear Cells (PBMCs) retrieved from four ADHD patients (two MPH responders and two non-responders) using Sendai virus. These lines might be helpful for the in vitro investigation of ADHD pathophysiology in a patient-specific manner.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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