Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution

Nicholas D. Leigh; Garrett S. Dunlap; Kimberly Johnson; Rachelle Mariano; Rachel Oshiro; Alan Y. Wong; Donald M. Bryant; Bess M. Miller; Alex Ratner; Andy Chen; William W. Ye; Brian J. Haas; Jessica L. Whited

doi:10.1038/s41467-018-07604-0

Nature Communications (Dec 2018)

Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution

Nicholas D. Leigh,
Garrett S. Dunlap,
Kimberly Johnson,
Rachelle Mariano,
Rachel Oshiro,
Alan Y. Wong,
Donald M. Bryant,
Bess M. Miller,
Alex Ratner,
Andy Chen,
William W. Ye,
Brian J. Haas,
Jessica L. Whited

Affiliations

Nicholas D. Leigh: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Garrett S. Dunlap: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Kimberly Johnson: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Rachelle Mariano: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Rachel Oshiro: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Alan Y. Wong: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Donald M. Bryant: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Bess M. Miller: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Alex Ratner: ICCB-L Single Cell Core, Harvard Medical School
Andy Chen: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
William W. Ye: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital
Brian J. Haas: Broad Institute of MIT and Harvard
Jessica L. Whited: Department of Orthopedic Surgery, Harvard Medical School, The Harvard Stem Cell Institute, Brigham and Women’s Hospital

DOI: https://doi.org/10.1038/s41467-018-07604-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 14

Abstract

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Limb regeneration requires a blastema with progenitor cells, immune cells, and an overlying wound epidermis, but molecular identities of these populations are unclear. Here, the authors use single-cell RNA-sequencing to identify transcriptionally distinct cell populations in adult axolotl limb blastemas.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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