Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae
Sviatlana Shashkova,
Adam Wollman,
Stefan Hohmann,
Mark C Leake
Affiliations
Sviatlana Shashkova
Biological Physical Science Institute, Departments of Physics and Biology, University of York, York, UKDepartment of Chemistry and Molecular Biology, University of Gothenburg, Göteborg, Sweden
Adam Wollman
Biological Physical Science Institute, Departments of Physics and Biology, University of York, York, UK
Stefan Hohmann
Department of Chemistry and Molecular Biology, University of Gothenburg, Göteborg, SwedenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden
Mark C Leake
Biological Physical Science Institute, Departments of Physics and Biology, University of York, York, UK
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here we present a method to characterise maturation of GFP and mCherry genetic protein fusions in budding yeast Saccharomyces cerevisiae.
