Comparison between the Effect of Advanced-Platelet Rich Fbrin and Leukocyte-Platelet Rich Fibrin on the Number of Fibroblasts Adhered to Collagen Membrane

Abstract

Growth factors and cytokines in the platelet playa major role inthe progression of inflammation and healing processes, therefore A-PRF and L-PRF as platelet drive ingredients are widely used in periodontal treatments. The purpose of this study is to compare the effect of A-PRF and L-PRF on the number of gingival fibroblastsadhered to collagen membrane.Materials & Methods:In this In-vitro study conductedin winter of 2021 at the Iranian biological resource center, the membrane was cut into five pieces (5x5mm) and placed at the bottom of a 5 well plate. 1 milliiiter of (A-PRF) was added to two wells ineach group. And a similar amount of L-PRF was added to two wells in theother two groups.The last well was left A-PRF or L-PRF free. Gingival fibroblasts were seeded inall the wells. Cell adhesion was evaluated by means of a Field Emission Scanning Electron Microscope after 24 hours of incubation. The Data wereanalyzed by, one way and two-way ANOVA and Scheffe test (p value < 0.001).Results:based on ANOVA there was a statistically significant difference In the A-PRF+ group and L-PRF+ group (12.50 and 13.57) compared to the control group (4.67) (p value < 0.001).and Scheffe analysis showed no statistically significant difference between A-PRF+ or L-PRF+ groups (p value = 0.307).Conclusion:A-PRF and L-PRF are both effective in the adhesion of fibroblasts cells to collagen membranes, although there is no difference between A-PRF and L-PRF in the adhesion of fibroblast cells to collagen membranes

Keywords

• membranes
• platelet rich fibrin
• fibroblasts