Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells

Muneaki Nakamura; Prashanth Srinivasan; Michael Chavez; Matthew A. Carter; Antonia A. Dominguez; Marie La Russa; Matthew B. Lau; Timothy R. Abbott; Xiaoshu Xu; Dehua Zhao; Yuchen Gao; Nathan H. Kipniss; Christina D. Smolke; Joseph Bondy-Denomy; Lei S. Qi

doi:10.1038/s41467-018-08158-x

Nature Communications (Jan 2019)

Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells

Muneaki Nakamura,
Prashanth Srinivasan,
Michael Chavez,
Matthew A. Carter,
Antonia A. Dominguez,
Marie La Russa,
Matthew B. Lau,
Timothy R. Abbott,
Xiaoshu Xu,
Dehua Zhao,
Yuchen Gao,
Nathan H. Kipniss,
Christina D. Smolke,
Joseph Bondy-Denomy,
Lei S. Qi

Affiliations

Muneaki Nakamura: Department of Bioengineering, Stanford University
Prashanth Srinivasan: Department of Bioengineering, Stanford University
Michael Chavez: Department of Bioengineering, Stanford University
Matthew A. Carter: Department of Bioengineering, Stanford University
Antonia A. Dominguez: Department of Bioengineering, Stanford University
Marie La Russa: Department of Bioengineering, Stanford University
Matthew B. Lau: Department of Bioengineering, Stanford University
Timothy R. Abbott: Department of Bioengineering, Stanford University
Xiaoshu Xu: Department of Bioengineering, Stanford University
Dehua Zhao: Department of Bioengineering, Stanford University
Yuchen Gao: Department of Bioengineering, Stanford University
Nathan H. Kipniss: Department of Bioengineering, Stanford University
Christina D. Smolke: Department of Bioengineering, Stanford University
Joseph Bondy-Denomy: Department of Microbiology and Immunology, University of California
Lei S. Qi: Department of Bioengineering, Stanford University

DOI: https://doi.org/10.1038/s41467-018-08158-x
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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Anti-CRISPR proteins derived from phage can abrogate CRISPR activity. The authors repurpose these molecules for demonstrating genomic write-protection and pre-programmed gene expression circuits.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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