Fabrication of Functional bioMOF-100 Prototype as Drug Delivery System for Breast Cancer Therapy
Renata Carolina Alves,
Richard Perosa Fernandes,
Renan Lira de Farias,
Patricia Bento da Silva,
Raquel Santos Faria,
Christian Rafael Quijia,
Regina Célia Galvão Frem,
Ricardo Bentes Azevedo,
Marlus Chorilli
Affiliations
Renata Carolina Alves
Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara Jau, Km 01—s/n—Campos Ville, Araraquara 14800-903, Brazil
Richard Perosa Fernandes
Department of Chemistry, Federal University of Mato Grosso (UFMT), Cuiabá 78060-900, Brazil
Renan Lira de Farias
Departament of Chemical, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro 22451-900, Brazil
Patricia Bento da Silva
Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia (UnB), Campus Universitario Darcy Ribeiro—Asa Norte, Brasilia 70910-900, Brazil
Raquel Santos Faria
Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia (UnB), Campus Universitario Darcy Ribeiro—Asa Norte, Brasilia 70910-900, Brazil
Christian Rafael Quijia
Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara Jau, Km 01—s/n—Campos Ville, Araraquara 14800-903, Brazil
Regina Célia Galvão Frem
Chemistry Institute, São Paulo State University (UNESP), Campus Araraquara, Araraquara 14800-060, Brazil
Ricardo Bentes Azevedo
Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia (UnB), Campus Universitario Darcy Ribeiro—Asa Norte, Brasilia 70910-900, Brazil
Marlus Chorilli
Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara Jau, Km 01—s/n—Campos Ville, Araraquara 14800-903, Brazil
Breast cancer is the most frequent cause of cancer death in women, representing the fifth leading cause of cancer death overall. Therefore, the growing search for the development of new treatments for breast cancer has been developed lately as well as drug delivery systems such as biocompatible metal–organic Frameworks (bio-MOFs). These may be promising and attractive for drug incorporation and release. The present study aims to develop a drug carrier system RCA (bioMOF-100 submitted to the activation process) containing incorporated curcumin (CCM), whose material surface is coated with folic acid molecules (FA) to promote the targeting of drug carrier systems to the tumor region. They were synthesized and characterized using several characterization techniques. The materials were submitted to drug encapsulation tests, whose encapsulation efficiency was 32.80% for CCM@RCA-1D. Using the 1H nuclear magnetic resonance (NMR) spectroscopy technique, it was possible to verify the appearance of signals referring to folic acid, suggesting success in the functionalization of these matrices. In vitro tests such as cell viability and type of cell death were evaluated in both series of compounds (CCM@RCA-1D, CCM@RCA-1D/FA) in breast tumor lines. The results revealed low toxicity of the materials and cell death by late apoptosis. Thus, these results indicate that the matrices studied can be promising carriers in the treatment of breast cancer.
