Determination of the Activity of the Anti-Rabies Sera and Heterologous Anti-Rabies Immunoglobulin <i>in vitro</i> in the Dot Immunoassay

Abstract

For the first time constructed was a diagnosticum based on colloid gold nanoparticles (with an average size of about 15-17 nm) to detect specific antibodies against rabies virus in the anti-rabies sera and immunoglobulin in vitro in the dot-immunoassay [24]. Investigated was the correlation between the results of the rabies virus neutralization test on white mice and the results of the dot immunoassay. The results of the dot immunoassay correlated with those of the neutralization test on white mice. Thus, the method offered can be considered as an alternative to the in vivo neutralization test. This in vitro method can be used to test the activity of the anti-rabies sera at the stage of horses immunization.

Keywords

• rabies
• nanoparticles
• colloid gold
• diagnosticum