Prevalence, antibiotic resistance and molecular characterization of Staphylococcus aureus in ready-to-eat fruits and vegetables in Shanghai, China

Abstract

Staphylococcus aureus (S. aureus) is one of the foodborne pathogens. This study aimed to investigate the prevalence of S. aureus in ready-to-eat (RTE) fruits and vegetables in Shanghai, China. We evaluated antibiotic resistance patterns and genetic diversity of isolates through whole genome sequencing. Our findings demonstrated that out of 143 market samples, 47 (32.87%) tested positive for S. aureus, with the prevalence rates ranging from 10% to 57.14% among 12 types of RTE fruits and vegetables. Most isolates were resistant to trimethoprim-sulphamethoxazole, oxacillin, and ampicillin. We identified a total of 15 antibiotic resistance genes associated with resistance to 6 antibiotics, such as fosfomycin, fluoroquinolone, and β-lactam. Adhesion genes and enterotoxin genes, including icaA, icaB, icaC, set, seg, and sec, were also identified. Seven multi-locus sequence types (MLST) were detected, two of which were novel (ST7208 and ST7986). Notably, ST705-t529 (34.04%) and ST6-t701 (27.79%) represented the predominant types of S. aureus. Furthermore, three of the isolates were confirmed to be methicillin-resistant S. aureus by mecA genes. Taken together, our results highlight the high prevalence of S. aureus in RTE fruits and vegetables, posing a potential threat to food safety, particularly due to its high level of antibiotic resistance.

Keywords

• Staphylococcus aureus
• Ready-to-eat fruits and vegetables
• Prevalence
• Antibiotic resistance
• Whole genome sequencing