Effect of Garlic and Alcoholic Extract on the Mitrogenic Soralene Antifungal in Mushrooms Aspergillus amstelodami

Abstract

This study includes testing the effect of four subinhibitory concentrations of garlic extracts (aqueous and alcoholic) on the mutagenicity of 8-methoxypsoralen (8-MOP) in the presence of long wave ultraviolet (UV A) in conidia of the fungus Aspergillus amstelodami .The concentrations of aqueous extract were used in this study are (2.5 , 5 , 7.5 and 10 mg I ml media ) and the concentrations of alcoholic extracts are ( 0.5 , 1 , 1.5 and 2 mg I ml media) . Two protocols were used to study the effect of garlic extracts on the mutagenicity of 8- MOP in the presence of UV A (366 nm) . In first protocol , the pretreatment method , all the concentrations (2.5 , 5 , 7.5 and 10 mg I ml )of aqueous extract increased the mutants frequencies which were induced with 8-MOP + UV A, but only the low concentration (0.5 mg I ml) of the alcoholic extract increases the frequency of the mutants induced with 8-MOP + UV A . In the second protocol , the growth mediated method , both the aqueous and alcoholic extracts showed inhibitory effect on the mutagenicity of 8-MOP + UV A.

Keywords

• garlic
• alcoholic
• mitrogenic soralene