Infectious Focus Assays and Multiplicity of Infection (MOI) Calculations for Alpha-herpesviruses

Abstract

Titration of viral stocks is a critical process before any experimental use of the virus. Here we describe an infectious focus assay for several alphaherpesviruses, a titration method for fluorescently labeled viruses, based on the original plaque assay. In addition, the calculation of multiplicity of infection (MOI) is presented.