Nature Communications (Dec 2022)
Force-tuned avidity of spike variant-ACE2 interactions viewed on the single-molecule level
- Rong Zhu,
- Daniel Canena,
- Mateusz Sikora,
- Miriam Klausberger,
- Hannah Seferovic,
- Ahmad Reza Mehdipour,
- Lisa Hain,
- Elisabeth Laurent,
- Vanessa Monteil,
- Gerald Wirnsberger,
- Ralph Wieneke,
- Robert Tampé,
- Nikolaus F. Kienzl,
- Lukas Mach,
- Ali Mirazimi,
- Yoo Jin Oh,
- Josef M. Penninger,
- Gerhard Hummer,
- Peter Hinterdorfer
Affiliations
- Rong Zhu
- Department of Experimental Applied Biophysics, Johannes Kepler University Linz
- Daniel Canena
- Department of Experimental Applied Biophysics, Johannes Kepler University Linz
- Mateusz Sikora
- Department of Theoretical Biophysics, Max Planck Institute of Biophysics
- Miriam Klausberger
- Department of Biotechnology, Institute of Molecular Biotechnology, University of Natural Resources and Life Sciences, Vienna
- Hannah Seferovic
- Department of Experimental Applied Biophysics, Johannes Kepler University Linz
- Ahmad Reza Mehdipour
- Department of Theoretical Biophysics, Max Planck Institute of Biophysics
- Lisa Hain
- Department of Experimental Applied Biophysics, Johannes Kepler University Linz
- Elisabeth Laurent
- Department of Biotechnology, Institute of Molecular Biotechnology, University of Natural Resources and Life Sciences, Vienna
- Vanessa Monteil
- Department of Laboratory Medicine, Unit of Clinical Microbiology, Karolinska Institute and Karolinska University Hospital
- Gerald Wirnsberger
- Apeiron Biologics
- Ralph Wieneke
- Institute of Biochemistry, Biocenter, Goethe University Frankfurt
- Robert Tampé
- Institute of Biochemistry, Biocenter, Goethe University Frankfurt
- Nikolaus F. Kienzl
- Department of Applied Genetics and Cell Biology, Institute of Plant Biotechnology and Cell Biology, University of Natural Resources and Life Sciences, Vienna
- Lukas Mach
- Department of Applied Genetics and Cell Biology, Institute of Plant Biotechnology and Cell Biology, University of Natural Resources and Life Sciences, Vienna
- Ali Mirazimi
- Department of Laboratory Medicine, Unit of Clinical Microbiology, Karolinska Institute and Karolinska University Hospital
- Yoo Jin Oh
- Department of Experimental Applied Biophysics, Johannes Kepler University Linz
- Josef M. Penninger
- Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
- Gerhard Hummer
- Department of Theoretical Biophysics, Max Planck Institute of Biophysics
- Peter Hinterdorfer
- Department of Experimental Applied Biophysics, Johannes Kepler University Linz
- DOI
- https://doi.org/10.1038/s41467-022-35641-3
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 17
Abstract
Combining high-speed AFM, single molecule recognition force spectroscopy, and molecular dynamics simulations Zhu, Canena, Sikora et al. characterize the interaction dynamics of the trimeric spike protein of SARS-CoV-2 wt, and delta and omicron variants with its entry receptor ACE2. While delta variant increases avidity by multivalent binding to ACE2, omicron variant shows an extended binding lifetime.
