Nature Communications (Jan 2022)
Towards a generic prototyping approach for therapeutically-relevant peptides and proteins in a cell-free translation system
- Yue Wu,
- Zhenling Cui,
- Yen-Hua Huang,
- Simon J. de Veer,
- Andrey V. Aralov,
- Zhong Guo,
- Shayli V. Moradi,
- Alexandra O. Hinton,
- Jennifer R. Deuis,
- Shaodong Guo,
- Kai-En Chen,
- Brett M. Collins,
- Irina Vetter,
- Volker Herzig,
- Alun Jones,
- Matthew A. Cooper,
- Glenn F. King,
- David J. Craik,
- Kirill Alexandrov,
- Sergey Mureev
Affiliations
- Yue Wu
- Institute for Molecular Bioscience, The University of Queensland
- Zhenling Cui
- ARC Centre of Excellence in Synthetic Biology
- Yen-Hua Huang
- Institute for Molecular Bioscience, The University of Queensland
- Simon J. de Veer
- Institute for Molecular Bioscience, The University of Queensland
- Andrey V. Aralov
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Zhong Guo
- ARC Centre of Excellence in Synthetic Biology
- Shayli V. Moradi
- ARC Centre of Excellence in Synthetic Biology
- Alexandra O. Hinton
- Institute for Molecular Bioscience, The University of Queensland
- Jennifer R. Deuis
- Institute for Molecular Bioscience, The University of Queensland
- Shaodong Guo
- Institute for Molecular Bioscience, The University of Queensland
- Kai-En Chen
- Institute for Molecular Bioscience, The University of Queensland
- Brett M. Collins
- Institute for Molecular Bioscience, The University of Queensland
- Irina Vetter
- Institute for Molecular Bioscience, The University of Queensland
- Volker Herzig
- Institute for Molecular Bioscience, The University of Queensland
- Alun Jones
- Institute for Molecular Bioscience, The University of Queensland
- Matthew A. Cooper
- Institute for Molecular Bioscience, The University of Queensland
- Glenn F. King
- Institute for Molecular Bioscience, The University of Queensland
- David J. Craik
- Institute for Molecular Bioscience, The University of Queensland
- Kirill Alexandrov
- ARC Centre of Excellence in Synthetic Biology
- Sergey Mureev
- School of Biology and Environmental Science, Queensland University of Technology
- DOI
- https://doi.org/10.1038/s41467-021-27854-9
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 17
Abstract
Generic approach for rapid prototyping is essential for the progress of synthetic biology. Here the authors modify the cell-free translation system to control protein aggregation and folding and validate the approach by using single conditions for prototyping of various disulfide-constrained polypeptides.
