Two-dimensional thin-layer chromatography of rat liver phosphatides

Abstract

A system of two-dimensional thin-layer chromatography was developed that separated rat liver phosphatides into several phosphate-positive spots in about 2 hr developing time. Characteristic hydrolysis products derived from phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl choline, sphingomyelin, and lysophosphatidyl choline were identified. The hydrolytic products of “phosphatidic acid” were not definitely characterized. The application of thin-layer chromatography as described for rat liver phosphatides can be extended to phosphatide extracts of other tissues.