Nature Communications (Sep 2018)
Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer
- Liwei Cao,
- Matthias Pauthner,
- Raiees Andrabi,
- Kimmo Rantalainen,
- Zachary Berndsen,
- Jolene K. Diedrich,
- Sergey Menis,
- Devin Sok,
- Raiza Bastidas,
- Sung-Kyu Robin Park,
- Claire M. Delahunty,
- Lin He,
- Javier Guenaga,
- Richard T. Wyatt,
- William R. Schief,
- Andrew B. Ward,
- John R. Yates,
- Dennis R. Burton,
- James C. Paulson
Affiliations
- Liwei Cao
- Department of Molecular Medicine, The Scripps Research Institute
- Matthias Pauthner
- Department of Immunology and Microbiology, The Scripps Research Institute
- Raiees Andrabi
- Department of Immunology and Microbiology, The Scripps Research Institute
- Kimmo Rantalainen
- Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute
- Zachary Berndsen
- Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute
- Jolene K. Diedrich
- Department of Molecular Medicine, The Scripps Research Institute
- Sergey Menis
- Department of Immunology and Microbiology, The Scripps Research Institute
- Devin Sok
- Department of Immunology and Microbiology, The Scripps Research Institute
- Raiza Bastidas
- Department of Immunology and Microbiology, The Scripps Research Institute
- Sung-Kyu Robin Park
- Department of Molecular Medicine, The Scripps Research Institute
- Claire M. Delahunty
- Department of Molecular Medicine, The Scripps Research Institute
- Lin He
- Department of Molecular Medicine, The Scripps Research Institute
- Javier Guenaga
- Department of Immunology and Microbiology, The Scripps Research Institute
- Richard T. Wyatt
- Department of Immunology and Microbiology, The Scripps Research Institute
- William R. Schief
- Department of Immunology and Microbiology, The Scripps Research Institute
- Andrew B. Ward
- Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute
- John R. Yates
- Department of Molecular Medicine, The Scripps Research Institute
- Dennis R. Burton
- Department of Immunology and Microbiology, The Scripps Research Institute
- James C. Paulson
- Department of Molecular Medicine, The Scripps Research Institute
- DOI
- https://doi.org/10.1038/s41467-018-06121-4
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 14
Abstract
HIV envelope (Env) is a potential vaccine antigen and its N-glycans are part of the epitope of broadly neutralizing antibodies. Here, the authors show that glycosylation of Env from infectious virus closely matches Env from recombinant membrane-bound trimers, while it differs significantly from recombinant soluble, cleaved Env trimers.
