Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

Abstract

Summary: This protocol describes the detailed procedures for utilizing human pluripotent stem cells (hPSCs) for pancreatic progenitor and hepatic differentiation, followed by the application of hPSC-derived cells in a luciferase reporter-based assay to study gene regulation. The generated hPSC-derived cells have been shown to achieve morphologies and gene expression profiles specific to their differentiated cell types, and subsequent luciferase assay has been shown to effectively elucidate the role of disease-relevant gene variants. Therefore, this protocol provides a valuable approach for pancreatic and liver disease modeling.For complete details on the use and execution of this protocol, please refer to Ng et al. (2019).

Keywords

• Cell culture
• Cell-based Assays
• Molecular Biology
• Gene Expression
• Stem Cells
• Cell Differentiation