Computational and Structural Biotechnology Journal (Dec 2024)

Transcriptomic profiling of Dip2a in the neural differentiation of mouse embryonic stem cells

  • Mingze Yao,
  • Lei Zhang,
  • Xiaojuan Teng,
  • Yu Lei,
  • Xiaoyu Xing,
  • Tinglin Ren,
  • Yuanqing Pan,
  • Liwen Zhang,
  • Zhengfeng Li,
  • Jingxia Lin,
  • Yaowu Zheng,
  • Li Xing,
  • Jiajian Zhou,
  • Changxin Wu

Journal volume & issue
Vol. 23
pp. 700 – 710

Abstract

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Introduction: The disconnected-interacting protein 2 homolog A (DIP2A), a member of disconnected-interacting 2 protein family, has been shown to be involved in human nervous system-related mental illness. This protein is highly expressed in the nervous system of mouse. Mutation of mouse DIP2A causes defects in spine morphology and synaptic transmission, autism-like behaviors, and defective social novelty [5,27], indicating that DIP2A is critical to the maintenance of neural development. However, the role of DIP2A in neural differentiation has yet to be investigated. Objective: To determine the role of DIP2A in neural differentiation, a neural differentiation model was established using mouse embryonic stem cells (mESCs) and studied by using gene-knockout technology and RNA-sequencing-based transcriptome analysis. Results: We found that DIP2A is not required for mESCs pluripotency maintenance, but loss of DIP2A causes the neural differentiation abnormalities in both N2B27 and KSR medium. Functional knockout of Dip2a gene also decreased proliferation of mESCs by perturbation of the cell cycle and profoundly inhibited the expression of a large number of neural development-associated genes which mainly enriched in spinal cord development and postsynapse assembly. Conclusions: The results of this report demonstrate that DIP2A plays an essential role in regulating differentiation of mESCs towards the neural fate.

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