EBioMedicine (Mar 2024)
In situ single-cell profiling sheds light on IFI27 localisation during SARS-CoV-2 infection
- Chin Wee Tan,
- Jinjin Chen,
- Ning Liu,
- Dharmesh D. Bhuva,
- Tony Blick,
- James Monkman,
- Caroline Cooper,
- Malvika Kharbanda,
- Kristen Feher,
- Belinda Phipson,
- Emily E. Killingbeck,
- Liuliu Pan,
- Youngmi Kim,
- Yan Liang,
- Andy Nam,
- Michael Leon,
- Paulo Souza-Fonseca-Guimaraes,
- Seigo Nagashima,
- Ana Paula Camargo Martins,
- Cleber Machado-Souza,
- Lucia de Noronha,
- Benjamin Tang,
- Kirsty Short,
- John Fraser,
- Gabrielle T. Belz,
- Fernando Souza-Fonseca-Guimaraes,
- Arutha Kulasinghe,
- Melissa J. Davis
Affiliations
- Chin Wee Tan
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia
- Jinjin Chen
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia
- Ning Liu
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; South Australian ImmunoGENomics Cancer Institute, The University of Adelaide, SA, Australia
- Dharmesh D. Bhuva
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; South Australian ImmunoGENomics Cancer Institute, The University of Adelaide, SA, Australia
- Tony Blick
- Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia
- James Monkman
- Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia
- Caroline Cooper
- Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia; Princess Alexandra Hospital, Woolloongabba, QLD, Australia
- Malvika Kharbanda
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; South Australian ImmunoGENomics Cancer Institute, The University of Adelaide, SA, Australia
- Kristen Feher
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; South Australian ImmunoGENomics Cancer Institute, The University of Adelaide, SA, Australia
- Belinda Phipson
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia
- Emily E. Killingbeck
- Nanostring Technologies, Inc, Seattle, WA, USA
- Liuliu Pan
- Nanostring Technologies, Inc, Seattle, WA, USA
- Youngmi Kim
- Nanostring Technologies, Inc, Seattle, WA, USA
- Yan Liang
- Nanostring Technologies, Inc, Seattle, WA, USA
- Andy Nam
- Nanostring Technologies, Inc, Seattle, WA, USA
- Michael Leon
- Nanostring Technologies, Inc, Seattle, WA, USA
- Paulo Souza-Fonseca-Guimaraes
- Pontifícia Universidade Católica do Paraná, PUCPR, Curitiba, Paraná, Brazil
- Seigo Nagashima
- Laboratório de Patologia Experimental, PPGCS da PUCPR, Curitiba, Brazil
- Ana Paula Camargo Martins
- Laboratório de Patologia Experimental, PPGCS da PUCPR, Curitiba, Brazil
- Cleber Machado-Souza
- Faculdades Pequeno Príncipe (FPP), Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), R. Silva Jardim, 1632-ÁguaVerde, Curitiba, 80230-020, PR, Brazil
- Lucia de Noronha
- Laboratório de Patologia Experimental, PPGCS da PUCPR, Curitiba, Brazil
- Benjamin Tang
- Westmead Institute for Medical Research, Sydney, Australia
- Kirsty Short
- School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia
- John Fraser
- Critical Care Research Group, The Prince Charles Hospital, Brisbane, Australia; Faculty of Medicine University of Queensland, Brisbane, QLD, Australia; St Andrew's War Memorial Hospital, UnitingCare, Spring Hill, QLD, Australia
- Gabrielle T. Belz
- Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia
- Fernando Souza-Fonseca-Guimaraes
- Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia
- Arutha Kulasinghe
- Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia; Corresponding author. Frazer Institute, Faculty of Medicine, Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia.
- Melissa J. Davis
- Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia; South Australian ImmunoGENomics Cancer Institute, The University of Adelaide, SA, Australia; Department of Clinical Pathology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia
- Journal volume & issue
-
Vol. 101
p. 105016
Abstract
Summary: The utilization of single-cell resolved spatial transcriptomics to delineate immune responses during SARS-CoV-2 infection was able to identify M1 macrophages to have elevated expression of IFI27 in areas of infection.
