Alcian blue as a kidney staining in diabetic mice

Abstract

One of the complications of Diabetes Mellitus (DM) is damage to the kidneys. Using L. plantarum, it can reduce glucose levels so that it is also possible to repair damaged kidney structures. The process of repairing the kidney structure by L plantarum can be seen microscopically in the glomerulus with Alcian Blue staining (AB). The purpose of the study was to determine the differences in the microscopic results of DM rat kidneys after treatment of L. plantarum with AB staining. The study sample used the kidneys of DM mice. The kidney of DM mice that were given L. plantarum at a dose of 1.0 ml/mice in each treatment, T1 (dose 1×1 times a day), T2 (dose 1×2 times a day), T3 (dose 1×3 times a day), then performed AB staining. The results of cytoplasmic cells in the glomerulus in negative controls/sick mice are stained dark blue/concentrated (strong intensity) with a score of 4, normal controls/healthy mice are not stained blue (negative) with a score of 1. T1 is a stained light blue score of 3, T2 is a stained light blue score of 3, and T3 is a colored pale blue score of 2. Based on these results, it was found that the color intensity of the AB kidney treatment of DM rats was the best in reducing glucose residues.

Keywords

• alcian blue staining
• lactobacillus plantarum
• diabetes mellitus
• diabetes mellitus treatment