Infrared Raman spectroscopy enables noninvasive biochemical assessment of skin tissue and the thermal stability
Xiaoyi Wang,
Fangqi Hua,
Xianhai Xie,
Yungang Wu,
Wenxue Sun,
Zipei Jiang,
Jinzhong Zhang,
Xu Luo
Affiliations
Xiaoyi Wang
Traditional Chinese Medicine (TCM) Orthopedics & Traumatology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
Fangqi Hua
Department of Wound Repair, the Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China
Xianhai Xie
Department of the Trauma, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
Yungang Wu
Traditional Chinese Medicine (TCM) Orthopedics & Traumatology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
Wenxue Sun
Hemodialysis Room, Department of Nephrology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
Zipei Jiang
Department of Ophthalmology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
Jinzhong Zhang
Department of Wound Repair, the Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China; Corresponding author.
Xu Luo
National Key Clinical Specialty (Wound Healing), Department of Wounds and Burns, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China; Key Laboratory of Intelligent Treatment and Life Support for Critical Diseases of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; Zhejiang Engineering Research Center for Hospital Emergency and Process Digitization, Wenzhou 325000, Zhejiang, China; Corresponding author at: National Key Clinical Specialty (Wound Healing), Department of Wounds and Burns, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
Raman-active modes of human skin and pork belly have been studied systematically by a near-infrared Raman spectrometer with an exciting laser of 1064nm. The main components and quantitative determination of pork belly are extracted by fitting the Raman spectra with the normalized Raman spectra of biochemical reagents such as collagen, elastin, triolein, fibronectin, fibrin, and hyaluronic acid. It demonstrates that the main components and quantity are various at different locations of pork belly, while the main components of human skin are similar to those of pig skin. In a further step, the evolution of the heating time-dependent Raman modes of isolated pig skin has been investigated for the mechanism of burnt skin. One can find that the spatial structure and main components of skin have an excellent thermal stability in the temperature range from -120 to 200∘C, which is confirmed by the temperature dependent Raman spectra of isolated pig skin, microporous acellular dermal matrix (MADM) as well as their corresponding biochemical reagents (collagen, elastin, triolein, etc.). These results help understand the mechanism of the living skin burnt by fire or hot water, and supplies an alternative technology for surgeons to diagnose the depth of a burn injury in time.
