The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from <i>Laurencia undulata</i> on Alcohol-Induced Hepatotoxicity in HepG2 Cells

Shengtao Yang; Mei-Fang Chen; Bomi Ryu; Jiali Chen; Zhenbang Xiao; Pengzhi Hong; Shengli Sun; Di Wang; Zhong-Ji Qian; Chunxia Zhou

doi:10.3390/molecules25051024

Molecules (Feb 2020)

The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from <i>Laurencia undulata</i> on Alcohol-Induced Hepatotoxicity in HepG2 Cells

Shengtao Yang,
Mei-Fang Chen,
Bomi Ryu,
Jiali Chen,
Zhenbang Xiao,
Pengzhi Hong,
Shengli Sun,
Di Wang,
Zhong-Ji Qian,
Chunxia Zhou

Affiliations

Shengtao Yang: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Mei-Fang Chen: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Bomi Ryu: Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea
Jiali Chen: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Zhenbang Xiao: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Pengzhi Hong: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Shengli Sun: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Di Wang: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Zhong-Ji Qian: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
Chunxia Zhou: College of Food Science and Technology, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China

DOI: https://doi.org/10.3390/molecules25051024
Journal volume & issue: Vol. 25, no. 5
p. 1024

Abstract

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Alcoholic liver disease (ALD) threatens human health, so it is imperative that we find ways to prevent or treat it. In recent years, the study of polysaccharides has shown that they have different kinds of bioactivities. Among them are many biological effects that have been attributed to polysaccharide precursors. D-Isofloridoside (DIF) is one of the polysaccharide precursors from the marine red alga Laurencia undulata. This study evaluated the effect of DIF on alcohol-induced oxidative stress in human hepatoma cells (HepG2). As a result, DIF attenuated alcohol-induced cytotoxicity, reduced the amount of intracellular reactive oxygen species (ROS), and effectively reduced alcohol-induced DNA damage in HepG2 cells. In addition, a western blot showed that, after DIF treatment, the expression levels of glutathione (GSH), superoxide dismutase (SOD), and B-cell lymphoma-2 (bcl-2) increased, while the expression levels of γ-glutamyl transferase (GGT), BCL2-associated X (bax), cleaved caspase-3, and mitogen-activated protein kinase (p38 and c-Jun N-terminal kinase) signal transduction proteins reduced. This showed that DIF may protect cells by reducing the amount of intracellular ROS and inhibiting intracellular oxidative stress and apoptotic processes. Finally, molecular docking demonstrated that DIF can bind to SOD, GGT, B-cell lymphoma-2, and bax proteins. These results indicated that DIF can protect HepG2 cells from alcohol-induced oxidative stress damage, making it an effective potential ingredient in functional foods.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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