Frontiers in Plant Science (Jan 2024)

A fast and easy one-step purification strategy for plant-made antibodies using Protein A magnetic beads

  • Loïc Faye,
  • Clemens Grünwald-Gruber,
  • Louis-Philippe Vezina,
  • Véronique Gomord,
  • Véronique Gomord,
  • Bertrand Morel

DOI
https://doi.org/10.3389/fpls.2023.1276148
Journal volume & issue
Vol. 14

Abstract

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A major difficulty to reach commercial- scale production for plant-made antibodies is the complexity and cost of their purification from plant extracts. Here, using Protein A magnetic beads, two monoclonal antibodies are purified in a one-step procedure directly from non-clarified crude plant extracts. This technique provides significant savings in terms of resources, operation time, and equipment.

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